FORMULATION AND PROTEIN ANALYSIS OF FISH FEED INCORPORATED
WITH ANNATTO SEEDS
I.W. Ofosu(1), M. Yeboah
Awudzi(1)* and J. K. Dadson(2).
(1) Department of Food Science and
Technology, Kwame Nkrumah University of Science and
Technology, Kumasi, Ghana. Department of Biochemistry and Biotechnology, Kwame
Nkrumah University of Science and Technology, Kumasi, Ghana.
The major operational cost in fish farming wh
ich is mostly feed, accounts for 50
70% of the variable cost depending on farming intensity. The rise in cost of commercial
feed is due to fish meal which is obtained from animal sources, hence there is the need to
alternative protein sources such as plant. In this work, feeds using annatto seeds and
soya bean meal as the protein source together with cassava flour and wheat bran were
formulated statistically. Crude protein analysis was run on each formulated sample
Kjedahl method and the data obtained was optimized in the statistical mixture design to study
the optimum crude protein. The optimum crude protein as proposed by the model was 18.81 %.
This was verified in the laboratory and crude protein of 18.71 %
was obtained, which was
close to that proposed by the model, suggesting that the data obtained fitted the quadratic
standard deviations of 0.65 and R2 of 0.79). A statistically formulated fish feed
based on annatto seed and soya bean meal
as a protein source was optimized with 25 % annatto
seeds, 20.49 % soya bean meal, 29.18 % cassava flour and 25.34% wheat bran as composition
with an appreciable crude protein content of 18.71 % obtained.
KEYWORDS: Crude protein, annatto seeds, soya bean
, fish feed